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Gewählte Publikation:

Saravia, V; Küpcü, S; Nolte, M; Huber, C; Pum, D; Fery, A; Sleytr, UB; Toca-Herrera, JL.
(2007): Bacterial protein patterning by micro-contact printing of PLL-g-PEG.
J Biotechnol. 2007; 130(3):247-252 FullText FullText_BOKU

Abstract:
Biomimetic micro-patterned surfaces of three S-layer (fusion) proteins, wild type (SbpA), enhanced green fluorescence protein (SbpA-EGFP) and streptavidin (SbpA-STV), were built by microcontact printing of poly-L-lysine grafted polyethylene glycol (PLL-g-PEG). The functionality of the adsorbed proteins was studied with atomic force microscopy and fluorescence microscopy. Atomic force microscopy (AFM) measurements showed that wild-type SbpA recrystallized on PLL-g-PEG free areas, while fluorescent properties of SbpA-EGFP and the interaction of SbpA-streptavidin heterotetramers with biotin were not affected due to the adsorption on the micro patterned substrates. (c) 2007 Published by Elsevier B.V.
Autor*innen der BOKU Wien:
Küpcü Seta
Pum Dietmar
Sleytr Uwe B.
Toca-Herrera José Luis
Find related publications in this database (using NML MeSH Indexing)
Bacterial Proteins - metabolism
Biotechnology - methods
Biotin - metabolism
Microscopy, Atomic Force -
Microscopy, Fluorescence -
Monosaccharide Transport Proteins - metabolism
Polyethylene Glycols - metabolism
Polylysine - metabolism
Recombinant Fusion Proteins - metabolism
Streptavidin - metabolism

Find related publications in this database (Keywords)
S-layer fusion protein
micro patterning
enhanced green fluorescent protein
streptavidin
atomic force microscopy
biomimetic surfaces


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