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Gewählte Publikation:

Gach, JS; Quendler, H; Strobach, S; Katinger, H; Kunert, R.
(2008): Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2F5.
Mol Immunol. 2008; 45(4):1027-1034 FullText FullText_BOKU

Abstract:
Anti-idiotypic antibody (Ab2) 3H6 is directed against the human monoclonal antibody 2175, which is one of a few neutralising antibodies against HIV-1. Since the binding epitope of 2175 is cryptic and no neutralising immune response could be elicited by several potential vaccines comprising this region, Ab2/3H6 represents a potent vaccine candidate for active immunisation. Here we describe the molecular features of Ab2/3H6 after changing the antigen binding specificity by single point mutations in the complementarity-determining region 3 of the Ab2/3H6 heavy chain. The resulting Ab2/3H6 mutants were compared in several experimental settings to the wild type Ab2/3H6 Fab fragment. Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice. (c) 2007 Elsevier Ltd. All rights reserved.
Autor*innen der BOKU Wien:
Katinger Hermann
Kunert Renate
Quendler Heribert
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies, Anti-Idiotypic - administration & dosage
Antibodies, Monoclonal - immunology
Antibody Formation -
CHO Cells -
Complementarity Determining Regions - administration & dosage
Cricetinae -
Cricetulus -
Epitopes -
Female -
Humans -
Immunoglobulin Fab Fragments - administration & dosage
Immunoglobulin Heavy Chains - administration & dosage
Mice -
Mice, Inbred BALB C -
Point Mutation -

Find related publications in this database (Keywords)
2F5
HIV-1
anti-idiotypic antibodies
vaccine
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