BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Kittl, R; Sygmund, C; Halada, P; Volc, J; Divne, C; Haltrich, D; Peterbauer, CK; .
(2008):
Molecular cloning of three pyranose dehydrogenase-encoding genes from Agaricus meleagris and analysis of their expression by real-time RT-PCR.
Curr Genet. 2008; 53(2):117-127
FullText
FullText_BOKU
- Abstract:
- Sugar oxidoreductases such as cellobiose dehydrogenase or pyranose oxidase are widespread enzymes among fungi, whose biological function is largely speculative. We investigated a similar gene family in the mushroom Agaricus meleagris and its expression under various conditions. Three genes (named pdh1, pdh2 and pdh3) putatively encoding pyranose dehydrogenases were isolated. All three genes displayed a conserved structure and organization, and the respective cDNAs contained ORFs translating into polypeptides of 602 or 600 amino acids. The N-terminal sections of all three genes encode putative signal peptides consistent with the enzymes extracellular secretion. We cultivated the fungus on different carbon sources and analyzed the mRNA levels of all three genes over a period of several weeks using real-time RT-PCR. The glyceraldehyde-3-phosphate dehydrogenase gene from A. meleagris was also isolated and served as reference gene. pdh2 and pdh3 are essentially transcribed constitutively, whereas pdh1 expression is upregulated upon exhaustion of the carbon source; pdh1 appears to be additionally regulated under conditions of oxygen limitation. These data are consistent with an assumed role in lignocellulose degradation.
- Autor*innen der BOKU Wien:
- Haltrich Dietmar
- Kittl Roman
- Peterbauer Clemens Karl
- Sygmund Christoph
- Find related publications in this database (using NML MeSH Indexing)
- Agaricus/genetics*;Carbohydrate Dehydrogenases/genetics*;Cellulose/metabolism;Cloning, Molecular;Fungal Proteins/genetics;Gene Expression Regulation, Fungal*;Genes, Fungal*;Lignin/metabolism;Membrane Proteins/genetics;Polymerase Chain Reaction;RNA, Messenger/analysis;
- Find related publications in this database (Keywords)
- pyranose dehydrogenase
- GMC-family of flavin-containing enzymes
- lignocellulose degradation
- agaricus spp
- transcription analysis
Altmetric: