BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Krska, R; Schubert-Ullrich, P; Molinelli, A; Sulyok, M; MacDonald, S; Crews, C.
(2008): Mycotoxin analysis: an update.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008; 25(2):152-163 FullText FullText_BOKU

Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.
Autor*innen der BOKU Wien:
Krska Rudolf
Sulyok Michael
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Chromatography, High Pressure Liquid - methods
Enzyme-Linked Immunosorbent Assay - methods
Food Contamination - analysis
Humans -
Mycotoxins - analysis
Reference Standards -
Tandem Mass Spectrometry - methods

Find related publications in this database (Keywords)
sample preparation
chromatographic methods

© BOKU Wien Impressum