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Gewählte Publikation:

Oliveira, JE., Damiani, R., Vorauer-Uhl, K., Bartolini, P., Ribela, MT..
(2008): Influence of a Reduced CO(2) Environment on the Secretion Yield, Potency and N-Glycan Structures of Recombinant Thyrotropin from CHO Cells
MOL BIOTECHNOL, 39(2), 159-166; ISSN 1073-6085 FullText FullText_BOKU

A consistent increase of similar to 60% in the secretion yield of CHO-derived hTSH was observed by changing cell culture CO2 conditions from 5% CO2 to an air environment. The overall quality of the products obtained under both conditions was evaluated in comparison with a well-known biopharmaceutical (Thyrogen (R)). The N-glycans identified were of the complex type, presenting di-, tri- and tetra-antennary structures, sometimes fucosylated, 86-88% of the identified structures being sialylated at variable levels. The three most abundant structures were monosialylated glycans, representing similar to 69% of all identified forms in the three preparations. The main difference was found in terms of antennarity, with 8-10% more di-antennary structures obtained in the absence of CO2 and 7-9% more tri-antennary structures in its presence. No remarkable difference in charge isomers was observed between the three preparations, the isoelectric focusing profiles showing six distinct bands in the 5.39-7.35 p (I) under bar range. A considerably different distribution, with more forms in the acidic region, was observed, however, for two native pituitary preparations. All recombinant preparations showed a higher in vivo bioactivity when compared to native hTSH. Different production processes apparently do not greatly affect N-glycan structures, charge isomer distribution or bioactivity of CHO-derived hTSH.
Autor*innen der BOKU Wien:
Vorauer-Uhl Karola

Find related publications in this database (Keywords)
CO2 environment
culture conditions
in vivo bioassay
isoelectric focusing
N-glycan structures

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