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Gewählte Publikation:

Jin, C; Altmann, F; Strasser, R; Mach, L; Schähs, M; Kunert, R; Rademacher, T; Glössl, J; Steinkellner, H.
(2008): A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits.
Glycobiology. 2008; 18(3):235-241 FullText FullText_BOKU

Abstract:
A common argument against using plants as a production system for therapeutic proteins is their inability to perform authentic N-glycosylation. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex N-glycans as these nonmammalian N-glycan residues may provoke unwanted side effects in humans. In this study we have investigated the potential antigenicity of plant-type N-glycans attached to a human monoclonal antibody (2G12). Using glyco-engineered plant lines as expression hosts, four 2G12 glycoforms differing in the presence/absence of beta 1,2-xylose and core alpha 1,3-fucose were generated. Systemic immunization of rabbits with a xylose and fucose carrying 2G12 glycoform resulted in a humoral immune response to both N-glycan epitopes. Furthermore, IgE immunoblotting with sera derived from allergic patients revealed binding to plant-produced 2G12 carrying core alpha 1,3 fucosylated N-glycan structures. Our results provide evidence for the adverse potential of nonmammalian N-glycan modi. cations present on monoclonal antibodies produced in plants. This emphasizes the need for the use of glycoengineered plants lacking any potentially antigenic N-glycan structures for the production of plant-derived recombinant proteins intended for parenteral human application.
Autor*innen der BOKU Wien:
Altmann Friedrich
Glößl Josef
Kunert Renate
Mach Lukas
Steinkellner Herta
Strasser Richard
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies - blood
Antibodies, Monoclonal - biosynthesis
Antibody Specificity - immunology
Antigens, Plant - chemistry
Blotting, Western -
CHO Cells -
Carbohydrates - chemistry
Cell Line -
Cricetinae -
Cricetulus -
Cross Reactions -
Enzyme-Linked Immunosorbent Assay -
Female -
Fucose - analysis
Humans -
Immunoglobulin E - blood
Plant Leaves - chemistry
Plants, Genetically Modified - immunology
Rabbits -
Tobacco - genetics
Xylose - analysis

Find related publications in this database (Keywords)
anti-carbohydrate immune response
beta 1,2 xylose
core alpha 1,3 fucose
glyco-engineered plants
recombinant antibodies
N-glycosylation


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