BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Jin, C; Altmann, F; Strasser, R; Mach, L; Schähs, M; Kunert, R; Rademacher, T; Glössl, J; Steinkellner, H.
(2008):
A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits.
Glycobiology. 2008; 18(3):235-241
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- Abstract:
- A common argument against using plants as a production system for therapeutic proteins is their inability to perform authentic N-glycosylation. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex N-glycans as these nonmammalian N-glycan residues may provoke unwanted side effects in humans. In this study we have investigated the potential antigenicity of plant-type N-glycans attached to a human monoclonal antibody (2G12). Using glyco-engineered plant lines as expression hosts, four 2G12 glycoforms differing in the presence/absence of beta 1,2-xylose and core alpha 1,3-fucose were generated. Systemic immunization of rabbits with a xylose and fucose carrying 2G12 glycoform resulted in a humoral immune response to both N-glycan epitopes. Furthermore, IgE immunoblotting with sera derived from allergic patients revealed binding to plant-produced 2G12 carrying core alpha 1,3 fucosylated N-glycan structures. Our results provide evidence for the adverse potential of nonmammalian N-glycan modi. cations present on monoclonal antibodies produced in plants. This emphasizes the need for the use of glycoengineered plants lacking any potentially antigenic N-glycan structures for the production of plant-derived recombinant proteins intended for parenteral human application.
- Autor*innen der BOKU Wien:
- Altmann Friedrich
- Glößl Josef
- Kunert Renate
- Mach Lukas
- Steinkellner Herta
- Strasser Richard
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Antibodies - blood
- Antibodies, Monoclonal - biosynthesis
- Antibody Specificity - immunology
- Antigens, Plant - chemistry
- Blotting, Western -
- CHO Cells -
- Carbohydrates - chemistry
- Cell Line -
- Cricetinae -
- Cricetulus -
- Cross Reactions -
- Enzyme-Linked Immunosorbent Assay -
- Female -
- Fucose - analysis
- Humans -
- Immunoglobulin E - blood
- Plant Leaves - chemistry
- Plants, Genetically Modified - immunology
- Rabbits -
- Tobacco - genetics
- Xylose - analysis
- Find related publications in this database (Keywords)
- anti-carbohydrate immune response
- beta 1,2 xylose
- core alpha 1,3 fucose
- glyco-engineered plants
- recombinant antibodies
- N-glycosylation
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