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Gewählte Publikation:

Weiss, A; Jérôme, V; Freitag, R; Mayer, HK.
(2008): Diversity of the resident microbiota in a thermophilic municipal biogas plant.
Appl Microbiol Biotechnol. 2008; 81(1):163-173 FullText FullText_BOKU

Abstract:
Biogas plants continuously convert biological wastes mainly into a mixture of methane, CO2 and H2O-a conversion that is carried out by a consortium of bacteria and archaea. Especially in the municipal plants dedicated towards waste treatment, the reactor feed may vary considerably, exposing the resident microbiota to a changing variety of substrates. To evaluate how and if such changes influence the microbiology, an established biogas plant (6,600 m(3), up to 600 m(3) biogas per h) was followed over the course of more than 2 years via polymerase chain reaction-denaturing gradient gel electrophoresis of 16S rRNA genes and subsequent sequencing. Both the bacterial and the archaeal community remained stable over the investigation. Of the bacterial consortium, about half of the sequences were in decreasing order of occurrence: Thermoacetogenium sp., Anaerobaculum mobile, Clostridium ultunense, Petrotoga sp., Lactobacillus hammesii, Butyrivibrio sp., Syntrophococcus sucromutans, Olsenella sp., Tepidanaerobacter sp., Sporanaerobacter acetigenes, Pseudoramibacter alactolyticus, Lactobacillus fuchuensis or Lactobacillus sakei, Lactobacillus parabrevis or Lactobacillus spicheri and Enterococcus faecalis. The other half matched closely to ones from similar habitats (thermophilic anaerobic methanogenic digestion). The archaea consisted of Methanobrevibacter sp., Methanoculleus bourgensis, Methanosphaera stadtmanae, Methanimicrococcus blatticola and uncultured Methanomicrobiales. The role of these species in methane production is discussed.
Autor*innen der BOKU Wien:
Mayer Helmut
Find related publications in this database (using NML MeSH Indexing)
Archaea - classification
Bacteria - classification
Biodiversity -
Bioreactors - microbiology
Biotransformation -
Carbon Dioxide - metabolism
DNA, Archaeal - genetics
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Hot Temperature -
Methane - metabolism
Molecular Sequence Data -
Phylogeny -
RNA, Ribosomal, 16S - genetics
Sewage - microbiology
Water - metabolism

Find related publications in this database (Keywords)
Biogas plant
Cloning
DGGE
Methanogenic consortium
Microbiota


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