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Gewählte Publikation:

Petersson A., Domig K.J., Nagel P., Zollitsch W., Hagmüller W., Kneifel W..
(2009): Denaturing gradient gel electrophoresis (DGGE)-based monitoring of intestinal lactobacilli and bifidobacteria of pigs during a feeding trial.
ARCH ANIM NUTR, 63 (2), 112-116; ISSN 0003-942X FullText FullText_BOKU

The aim of this study was to determine the influence of different feeding strategies on the gut microbiota of organic growing-finishing pigs. A total of 76 pigs were allocated to four different dietary treatments (control, probiotics, maize silage and grass silage). Effects of the applied probiotic preparation on the composition of the intestinal and faecal microbiota were monitored. By using a DGGE (denaturing gradient gel electrophoresis)-based methodology, fingerprints of the intestinal microbiota were obtained. The total microbial DNA was isolated from faecal and colon samples and amplified with PCR using different primer sets to detect bifidobacteria and lactobacilli. PCR products were separated using DGGE and the resulting profiles were compared with the findings of the other dietary treatments. Bands were excised from the gels and sequenced for further identification. Particularly two different DGGE profiles of bifidobacteria were observed, while lactobacilli showed larger variety within the dietary treatments.
Autor*innen der BOKU Wien:
Domig Konrad
Kneifel Wolfgang
Petersson Agnes
Zollitsch Werner
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animal Feed/analysis*
Animal Nutritional Physiological Phenomena
Bifidobacterium/isolation & purification*
DNA, Bacterial/isolation & purification
Electrophoresis, Gel, Two-Dimensional/methods*
Gastrointestinal Contents/microbiology
Lactobacillus/isolation & purification*

Find related publications in this database (Keywords)
denaturing gradient gel electrophoresis

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