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Gewählte Publikation:

Arnold, GF; Velasco, PK; Holmes, AK; Wrin, T; Geisler, SC; Phung, P; Tian, Y; Resnick, DA; Ma, X; Mariano, TM; Petropoulos, CJ; Taylor, JW; Katinger, H; Arnold, E.
(2009): Broad neutralization of human immunodeficiency virus type 1 (HIV-1) elicited from human rhinoviruses that display the HIV-1 gp41 ELDKWA epitope.
J Virol. 2009; 83(10):5087-5100 FullText FullText_BOKU

Abstract:
In efforts to develop AIDS vaccine components, we generated combinatorial libraries of recombinant human rhinoviruses that display the well-conserved ELDKWA epitope of the membrane-proximal external region of human immunodeficiency virus type 1 (HIV-1) gp41. The broadly neutralizing human monoclonal antibody 2F5 was used to select for viruses whose ELDKWA conformations resemble those of HIV. Immunization of guinea pigs with different chimeras, some boosted with ELDKWA-based peptides, elicited antibodies capable of neutralizing HIV-1 pseudoviruses of diverse subtypes and coreceptor usages. These recombinant immunogens are the first reported that elicit broad, albeit modest, neutralization of HIV-1 using an ELDKWA-based epitope and are among the few reported that elicit broad neutralization directed against any recombinant HIV epitope, providing a critical advance in developing effective AIDS vaccine components.
Autor*innen der BOKU Wien:
Katinger Hermann
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
AIDS Vaccines - immunology
Animals -
Antibodies, Monoclonal - immunology
Antigens, Viral - immunology
Enzyme-Linked Immunosorbent Assay -
Epitopes - immunology
Guinea Pigs -
HIV Antibodies - immunology
HIV Envelope Protein gp41 - immunology
HIV-1 - immunology
Hela Cells -
Humans -
Immunoglobulin G - immunology
Male -
Neutralization Tests -
Peptide Library -
Protein Engineering -
Rhinovirus - genetics



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