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Gewählte Publikation:

Grzeskowiak, JK; Tscheliessnig, A; Toh, PC; Chusainow, J; Lee, YY; Wong, N; Jungbauer, A.
(2009): Two-dimensional fluorescence difference gel electrophoresis for comparison of affinity and non-affinity based downstream processing of recombinant monoclonal antibody.
J Chromatogr A. 2009; 1216(24):4902-4912 FullText FullText_BOKU

Although Staphylococcus Protein A (SpA) affinity chromatography is the state of the art capture step for antibody purification, non-affinity methods are more economical. We used two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) to evaluate the purification Of a recombinant IgG(1) antibody from cultured cells, with two different processes: (I) SpA capture followed by cation-exchange chromatography (CEX); and (2) CEX capture, followed by anion exchanger, then hydrophobic interaction chromatography. Efficiencies were similar in sodium dodecylsulphate polyacrylamide gel electrophoresis and size-exclusion chromatography; however, 2-D DIGE revealed higher efficiency with SpA than with CEX capture. Thus, 2-D DIGE is a Valuable tool for downstream process development. (C) 2009 Elsevier B.V. All rights reserved.
Autor*innen der BOKU Wien:
Jungbauer Alois
Tscheließnig Anne-Luise
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Antibodies, Monoclonal - chemistry
CHO Cells -
Chromatography - methods
Chromatography, Affinity -
Cricetinae -
Cricetulus -
Electrophoresis, Gel, Two-Dimensional - methods
Fluorescence -
Immunoglobulin G - chemistry
Recombinant Proteins - chemistry
Staphylococcal Protein A - chemistry

Find related publications in this database (Keywords)
Recombinant antibody
Staphylococcus Protein A chromatography
Affinity chromatography
Ion exchange chromatography

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