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Gewählte Publikation:

Raberger, J; Schebesta, A; Sakaguchi, S; Boucheron, N; Blomberg, KEM; Berglof, A; Kolbe, T; Smith, CIE; Rulicke, T; Ellmeier, W.
(2008): The transcriptional regulator PLZF induces the development of CD44 high memory phenotype T cells
PROC NAT ACAD SCI USA. 2008; 105(46): 17919-17924. FullText FullText_BOKU

Abstract:
Transcriptional pathways controlling the development of CD44(hi) memory phenotype (MP) T cells with "innate-like" functions are not well understood. Here we show that the BTB (bric-a-brac, tramtrack, broad complex) domain-containing protein promyelocytic leukemia zinc finger (PLZF) is expressed in CD44(hi), but not in CD44(lo), CD4(+) T cells. Transgenic expression of PLZF during T cell development and in CD4(+) and CD8(+) T cells induced a T cell intrinsic program leading to an increase in peripheral CD44(hi) MP CD4(+) and CD8(+) T cells and a corresponding decrease of naive CD44(lo) T cells. The MP CD4(+) and CD8(+) T cells produced IFN gamma upon PMA/ionomycin stimulation, thus showing innate-like function. Changes in the naive versus memory-like subset distribution were already evident in single-positive thymocytes, indicating PLZF-induced T cell developmental alterations. In addition, CD1d-restricted natural killer T cells in PLZF transgenic mice showed impaired development and were severely reduced in the periphery. Finally, after anti-CD3/CD28 stimulation, CD4(+) transgenic T cells showed reduced IL-2 and IFN gamma production but increased IL-4 secretion as a result of enhanced IL-4 production of the CD44(hi)CD62L(+) subset. Our data indicate that PLZF is a novel regulator of the development of CD44(hi) MP T cells with a characteristic partial innate-like phenotype.
Autor*innen der BOKU Wien:
Kolbe Thomas
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Antigens, CD44 - immunology
CD4-Positive T-Lymphocytes - cytology
Cytokines - metabolism
Immunologic Memory - immunology
Kruppel-Like Transcription Factors - metabolism
L-Selectin - metabolism
Mice -
Mice, Transgenic -
Natural Killer T-Cells - cytology
Phenotype -
Receptors, Antigen, T-Cell - metabolism
T-Lymphocyte Subsets - immunology
T-Lymphocytes - cytology
Thymus Gland - cytology

Find related publications in this database (Keywords)
innate-like lymphocytes
T cell development
transgenics


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