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Gewählte Publikation:

Pontiller, J; Gross, S; Thaisuchat, H; Hesse, F; Ernst, W.
(2008): Identification of CHO endogenous promoter elements based on a genomic library approach
MOL BIOTECHNOL. MOLECULAR BIOTECHNOLOGY; 39: 135-139. (ISBN: Sao Paulo, Brazil) FullText FullText_BOKU

Today the standard technology for the production of many biopharmaceutical products from mammalian cell systems is frequently based on expression vectors that utilize strong mammalian active viral promoters like CMV or SV40 for driving recombinant gene transcription. On one hand these promoters allow very high expression rates, but on the other hand, they can lead to constitutive over-expression of the gene of interest resulting in a permanent stress on the cell. Another drawback is that they are cell cycle-dependent and can be subject to gene silencing which leads to a heterogeneity within the cell population. Here we aim to identify endogenous gene regulatory elements that are capable of controlling the transcription of the foreign gene. For this purpose, a genomic CHO library containing fragments of various lengths was constructed using a shotgun cloning strategy. After enzymatic fragmentation of genomic DNA, fragments encoding potential promoter regions were inserted into a promoterless vector upstream of the neomycin resistance gene. This random pool of library plasmids was transfected back into CHO cells and via cell sorting directly into 96-well plates and G418 (neomycin) selection, positive clones were isolated. A nested PCR of resistant CHO clones resulted in potential fragments which were sequenced. Putative promoter activity was predicted for these sequences by in silico methods and will be proved by re-transfection of reporter constructs into CHO cells.
Autor*innen der BOKU Wien:
Ernst Wolfgang
Gross Stefan
Hesse Friedemann
Pontiller Jens
Find related publications in this database (using NML MeSH Indexing)
Animals -
Base Sequence -
CHO Cells -
Cloning, Molecular -
CpG Islands -
Cricetinae -
Cricetulus -
Flow Cytometry -
Genes, Reporter -
Genetic Vectors - biosynthesis
Genomic Library -
Molecular Sequence Data -
Plasmids - biosynthesis
Promoter Regions, Genetic -
Regulatory Elements, Transcriptional -
Software -
Transfection -

Find related publications in this database (Keywords)
Chinese hamster ovary
recombinant protein expression
promoter prediction
in silico
DNA fragmentation
neomycin selection

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