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Gewählte Publikation:

Jungbauer, A; Hahn, R.
(2004): Engineering protein A affinity chromatography
CURR OPIN DRUG DISCOV DEV. 2004; 7(2): 248-256.

Abstract:
Staphylococcal protein A can selectively interact with immunoglobulins. This protein is widely used as a ligand for affinity chromatography to purify therapeutic antibodies on an industrial scale. This type of affinity chromatography constitutes a generic step in processing antibodies. Questions of scale-up, design of chromatographic conditions, clearance of adventitious agents and operational modes, such as continuous operation or purification of antibodies in expanded-bed mode, will be addressed in this review.
Autor*innen der BOKU Wien:
Hahn Rainer
Jungbauer Alois
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Antibodies, Monoclonal/isolation & purification*;Chromatography, Affinity/methods*;Immunoglobulin G/isolation & purification;Ligands;Models, Chemical;Protein Engineering*;Staphylococcal Protein A/chemistry*;

Find related publications in this database (Keywords)
antibody
immunoglobulin
ligand leakage
purification
scale-up
virus removal


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