BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Radwan, M; Miller, I; Grunert, T; Marchetti-Deschmann, M; Vogl, C; O'Donoghue, N; Dunn, MJ; Kolbe, T; Allmaier, G; Gemeiner, M; Müller, M; Strobl, B.
(2008):
The impact of tyrosine kinase 2 (Tyk2) on the proteome of murine macrophages and their response to lipopolysaccharide (LPS).
Proteomics. 2008; 8(17):3469-3485
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- Abstract:
- Tyrosine kinase 2 (Tyk2) belongs to the Janus kinase (Jak) family and is involved in signalling via a number of cytokines. Tyk2-deficient mice are highly resistant to lipopolysaccharide (LPS)-induced endotoxin shock. Macrophages are key players in the pathogenesis of endotoxin shock and, accordingly, defects in the LPS responses of Tyk2(-/-) macrophages have been reported. In the present study, the molecular role of Tyk2 is investigated in more detail using a proteomics approach. 2-D DIGE was applied to compare protein patterns from wild-type and Tyk2(-/-) macrophages and revealed significant differences in protein expression patterns between the genotypes before and after LPS treatment. Twenty-one proteins deriving from 25 differentially expressed spots were identified by MALDI/ESI MS. Among them, we show for N-myc interactor that its mRNA transcription/stability is positively influenced by Tyk2. In contrast, LPS-induced expression of plasminogen activator 2 protein but not mRNA is strongly enhanced in the absence of Tyk2. Our data furthermore suggest an influence of Tyk2 on the subcellular distribution of elongation factor 2 and on LPS-mediated changes in the peroxiredoxin 1 spot pattern. Thus, our results imply regulatory roles of Tyk2 at multiple levels and establish novel connections between Tyk2 and several cellular proteins.
- Autor*innen der BOKU Wien:
- Kolbe Thomas
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Cell Cycle Proteins - biosynthesis
- Electrophoresis, Gel, Two-Dimensional -
- Female -
- Intracellular Signaling Peptides and Proteins -
- Lipopolysaccharides - pharmacology
- Macrophages - drug effects
- Male -
- Mice -
- Mice, Knockout -
- Peptide Elongation Factor 2 - biosynthesis
- Peroxiredoxins - biosynthesis
- Plasminogen Activator Inhibitor 2 - biosynthesis
- Proteome - drug effects
- RNA, Messenger - metabolism
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -
- TYK2 Kinase - deficiency
- Find related publications in this database (Keywords)
- 2-D DIGE
- Jaks
- LPS
- MS
- murine macrophages
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