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Gewählte Publikation:

Probst, AV; Fagard, M; Proux, F; Mourrain, P; Boutet, S; Earley, K; Lawrence, RJ; Pikaard, CS; Murfett, J; Furner, I; Vaucheret, H; Mittelsten Scheid, O.
(2004): Arabidopsis histone deacetylase HDA6 is required for maintenance of transcriptional gene silencing and determines nuclear organization of rDNA repeats.
Plant Cell. 2004; 16(4):1021-1034 FullText FullText_BOKU

Histone acetylation and deacetylation are connected with transcriptional activation and silencing in many eukaryotic organisms. Gene families for enzymes that accomplish these modifications show a surprising multiplicity in sequence and expression levels, suggesting a high specificity for different targets. We show that mutations in Arabidopsis (Arabidopsis thaliana) HDA6, a putative class I histone deacetylase gene, result in loss of transcriptional silencing from several repetitive transgenic and endogenous templates. Surprisingly, total levels of histone H4 acetylation are only slightly affected, whereas significant hyperacetylation is restricted to the nucleolus organizer regions that contain the rDNA repeats. This switch coincides with an increase of histone 3 methylation at Lys residue 4, a modified DNA methylation pattern, and a concomitant decondensation of the chromatin. These results indicate that HDA6 might play a role in regulating activity of rRNA genes, and this control might be functionally linked to silencing of other repetitive templates and to its previously assigned role in RNA-directed DNA methylation.
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Find related publications in this database (using NML MeSH Indexing)
Acetylation -
Alleles -
Amino Acid Sequence -
Arabidopsis - enzymology
Arabidopsis Proteins -
Base Sequence -
DNA Methylation -
DNA, Plant - genetics
DNA, Ribosomal - genetics
Gene Silencing -
Histone Deacetylases - genetics
Histones - chemistry
Molecular Sequence Data -
Mutation -
Plants, Genetically Modified -
Repetitive Sequences, Nucleic Acid -
Sequence Homology, Amino Acid -

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