BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Acosta, JAT; Werner, U; Bachmann, H; Berthiller, F; Schuhamacher, R; Krska, R; Hauser, MT; Adam, G.
(2008):
A yeast based screening method for arabidopsis zearalenone-metabolism mutants
CEREAL RES COMMUN. CEREAL RESEARCH COMMUNICATIONS; 36: 545-548.
- Abstract:
- Arabidopsis thaliana and other plants are able to rapidly metabolize the estrogenic Fusarium metabolite zearalenone (ZON) into ZON-glucoside and further unknown compounds. We present here a rapid and efficient method to screen for Arabidopsis mutants unable to metabolize ZON. Arabidopsis seedling are grown in 96 well microtiter plates and subsequently incubated with ZON and P-glucosidase. The remaining ZON in the supernatant is detected with an engineered estrogen-responsive yeast strain. Extracellular ZON-4-O-glucoside (ZON-Gle), which is not detected by the indicator strain, is converted back to ZON by the added almond beta-glucosidase. Wildtype Arabidopsis seedlings inactivate 2 ppm ZON in three days, which is easily detected by the colorimetric readout. The assay Should be useful to identify mutants affected in ZON metabolism.
- Autor*innen der BOKU Wien:
- Adam Gerhard
- Berthiller Franz
- Hauser Marie-Theres
- Krska Rudolf
- Schuhmacher Rainer
- Find related publications in this database (Keywords)
- arabidopsis
- biotransformation
- zearalenone
- yeast bioassay