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Gewählte Publikation:

Steinborn, R; Zakhartchenko, V; Jelyazkov, J; Klein, D; Wolf, E; Muller, M; Brem, G.
(1998): Composition of parental mitochondrial DNA in cloned bovine embryos
FEBS LETT. 1998; 426(3): 352-356.

Abstract:
We have investigated parental mitochondrial DNA (mtDNA) in cloned bovine embryos obtained by intraspecific cytoplast-blastomere fusion. Analysis of two-cell to blastocyst stage embryos revealed that in contrast to the exclusion of paternal (sperm) mtDNA during sexual inheritance in the cytoplast-blastomere fusion complexes, there was mixing and co-existence of parental mtDNA. The mixing of mtDNA was non-balanced with the minority deriving from the blastomere. The constant content of mtDNA during embryogenesis until the blastocyst stage suggesting an absence of mtDNA replication was shown for conventional 'in vitro fertilised' (IVF) embryos and for cloned embryos. The ratio of parental mtDNA was in accordance with the estimated quantitative participation of mtDNA from the fusion partners. (C) 1998 Federation of European Biochemical Societies.
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Find related publications in this database (Keywords)
cloning
bovine
mitochondrial DNA
fusion
allele-specific quantitation
TaqMan (TM) polymerase chain reaction


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