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Gewählte Publikation:

Bahl, H; Scholz, H; Bayan, N; Chami, M; Leblon, G; GulikKrzywicki, T; Shechter, E; Fouet, A; Mesnage, S; TosiCouture, E; Gounon, P; Mock, M; deMacario, EC; Macario, AJL; FernandezHerrero, LA; Olabarria, G; Berenguer, J; Blaser, MJ; Kuen, B; Lubitz, W; Sara, M; Pouwels, PH; Kolen, CPAM; Boot, HJ; Palva, A; Truppe, M; Howorka, S; Schroll, G; Lechleitner, S; Resch, S.
(1997): Molecular biology of S-layers
FEMS MICROBIOL REV. FEMS MICROBIOLOGY REVIEWS; 20: 47-98.

Abstract:
In this chapter we report on the molecular biology of crystalline surface layers of different bacterial groups. The limited information indicates that there are many variations on a common theme. Sequence variety, antigenic diversity, gene expression, rearrangements, influence of environmental factors and applied aspects are addressed. There is considerable variety in the S-layer composition, which was elucidated by sequence analysis of the corresponding genes. In Corynebacterium glutamicum one major cell wall protein is responsible for the formation of a highly ordered, hexagonal array. In contrast, two abundant surface proteins form the S-layer of Bacillus anthracis. Each protein possesses three S-layer homology motifs and one protein could be a virulence factor. The antigenic diversity and ABC transporters are important features, which have been studied in methanogenic archaea. The expression of the S-layer components is controlled by three genes in the case of Thermus thermophilus. One has repressor activity on the S-layer gene promoter, the second codes for the S-layer protein. The rearrangement by reciprocal recombination was investigated in Campylobacter fetus. 7-8 S-layer proteins with a high degree of homology at the 5' and 3' ends were found. Environmental changes influence the surface properties of Bacillus stearothermophilus. Depending on oxygen supply, this species produces different S-layer proteins. Finally, the molecular bases for some applications are discussed. Recombinant S-layer fusion proteins have been designed for biotechnology.
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Find related publications in this database (Keywords)
Corynebacterium glutamicum
cspB gene
PS2 protein
S-layer
Bacillus anthracis
EA1 protein
Sap protein
SLH motif
methanogenic archaea
antigenic diversity
S-layer protein
ABC transporter
Methanosarcina mazei
Thermus thermophilus
gene regulation
Bacillus stearothermophilus
S-layer variation
oxygen stress
Lactobacilli
slp gene
Lactobacillus brevis
slpA gene
secretion of beta-lactamase
S-layer gene
sbs gene
heterologous expression
S-layer structure-function relationship
SbsA/SbsB fusion protein


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