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Gewählte Publikation:

Heinl, S; Hartinger, D; Thamhesl, M; Vekiru, E; Krska, R; Schatzmayr, G; Moll, WD; Grabherr, R; .
(2010): Degradation of fumonisin B1 by the consecutive action of two bacterial enzymes.
J Biotechnol. 2010; 145(2):120-129 FullText FullText_BOKU

Abstract:
Detoxification of the mycotoxin fumonisin B-1 comprises at least two enzymatic steps, an initial deesterification reaction, followed by deamination of the resulting hydrolyzed fumonisin B-1. In this study, two genes that are responsible for degradation of fumonisin B-1 by the bacterium Sphingopyxis sp. MTA144 were identified within a gene cluster, assumed to be associated with fumonisin degradation. The first gene encodes a protein which shows similarity to carboxylesterases, type B. The second gene encodes a polypeptide homologous to aminotransferases, class III. The two genes were isolated and expressed heterologously. The effect of the recombinant enzymes on fumonisin B-1 and hydrolyzed fumonisin B-1 was determined. The recombinant carboxylesterase was shown to catalyze the deesterification of fumonisin B-1 to hydrolyzed fumonisin B-1. The heterologously expressed aminotransferase was shown to deaminate hydrolyzed fumonisin B-1 in the presence of pyruvate and pyridoxal phosphate. We propose that the consecutive action of these two enzymes is sufficient for fumonisin B-1 detoxification. The results of this work provide a basis for the development of an enzymatic detoxification process for fumonisin B-1 in food and animal feed, especially under oxygen limited conditions, as they are found, e.g. in ensilaged forage or in the intestinal tract of animals. (C) 2009 Elsevier B.V. All rights reserved.
Autor*innen der BOKU Wien:
Grabherr Reingard
Heinl Stefan
Krska Rudolf
Kunz-Vekiru Elisavet
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Biodegradation, Environmental;Carboxylesterase/chemistry*;Carboxylesterase/metabolism*;Enzyme Activation;Fumonisins/chemistry*;Fumonisins/metabolism*;Hydrolysis;Sphingomonadaceae/enzymology*;Transaminases/chemistry*;Transaminases/metabolism*;

Find related publications in this database (Keywords)
Fumonisin B-1
HFB1
Aminotransferase
Deamination
Detoxification
Biodegradation


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