BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Meneely, JP; Sulyok, M; Baumgartner, S; Krska, R; Elliott, CT.
(2010):
A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food.
Talanta. 2010; 81(1-2):630-636
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- Abstract:
- A rapid surface plasmon resonance (SPR) screening assay has been developed for the combined detection of T-2 and HT-2 toxins in naturally contaminated cereals using a sensor chip coated with an HT-2 toxin derivative and a monoclonal antibody. The antibody raised against HT-2 displayed high cross-reactivity with T-2 toxin while there was no cross-reaction observed with other commonly occurring trichothecenes. A simple extraction procedure using 40% methanol was applied to baby food, breakfast cereal, and wheat samples prior to biosensor analysis. Limits of detection (LOD) for each matrix were determined as 25 mu g kg(-1) for baby food and breakfast cereal and 26 mu g kg(-1) for wheat. Intra-assay precision (n = 6) was calculated for each matrix. The results were expressed as the relative standard deviation and determined as 2.8% (100 mu g kg(-1)) and 1.8% (200 mu g kg(-1)) in breakfast cereal, 4.6% (50 mu g kg(-1)) and 3.6% (100 mu g kg(-1)) in wheat and 0.97% (25 mu g kg(-1)) and 6.3% (50 mu g kg(-1)) in baby food. Between run precision (n = 3) performed at the same levels yielded relative standard deviations of 6.7% and 3.9% for breakfast cereals, 3.3% and 1.6% for wheat and 6.8% and 0.08% for baby food, respectively. (C) 2010 Elsevier B.V. All rights reserved.
- Autor*innen der BOKU Wien:
- Baumgartner Sabine
- Krska Rudolf
- Sulyok Michael
- Find related publications in this database (using NML MeSH Indexing)
- Cereals - chemistry
- Food Analysis -
- Immunoassay - methods
- Infant Food - analysis
- Optical Processes -
- Reproducibility of Results -
- T-2 Toxin - analogs & derivatives
- Time Factors -
- Zea mays - chemistry
- Find related publications in this database (Keywords)
- Surface plasmon resonance
- Biacore (R) Q
- T-2 toxin
- HT-2 toxin
- Screening assay
- Semiquantitative
- Cereals
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