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Gewählte Publikation:

Banerjee, S; Zamocky, M; Furtmüller, PG; Obinger, C; .
(2010): Probing the two-domain structure of homodimeric prokaryotic and eukaryotic catalase-peroxidases.
Biochim Biophys Acta. 2010; 1804(11):2136-2145 FullText FullText_BOKU

Catalase-peroxidases (KatGs) are ancestral bifunctional heme peroxidases found in archaeons, bacteria and lower eukaryotes. In contrast to homologous cytochrome c peroxidase (CcP) and ascorbate peroxidase (APx) homodimeric KatGs have a two-domain monomeric structure with a catalytic N-terminal heme domain and a C-terminal domain of high sequence and structural similarity but without obvious function. Nevertheless, without its C-terminal counterpart the N-terminal domain exhibits neither catalase nor peroxidase activity. Except some hybrid-type proteins all other members of the peroxidase-catalase superfamily lack this C-terminal domain. In order to probe the role of the two-domain monomeric structure for conformational and thermal stability urea and temperature-dependent unfolding experiments were performed by using UV-Vis-, electronic circular dichroism- and fluorescence spectroscopy, as well as differential scanning calorimetry. Recombinant prokaryotic (cyanobacterial KatG from Synechocystis sp. PCC6803) and eukaryotic (fungal KatG from Magnaporthe grisea) were investigated. The obtained data demonstrate that the conformational and thermal stability of bifunctional KatGs is significantly lower compared to homologous monofunctional peroxidases. The N- and C-terminal domains do not unfold independently. Differences between the cyanobacterial and the fungal enzyme are relatively small. Data will be discussed with respect to known structure and function of KatG, CcP and APx. (C) 2010 Elsevier B.V. All rights reserved.
Autor*innen der BOKU Wien:
Banerjee Srijib
Furtmüller Paul Georg
Obinger Christian
Zamocky Marcel
Find related publications in this database (using NML MeSH Indexing)
Ascorbate Peroxidases;Calorimetry, Differential Scanning;Catalase/chemistry*;Cyanobacteria/chemistry*;Cytochrome-c Peroxidase/chemistry;Hot Temperature;Magnaporthe/metabolism;Peroxidase/chemistry*;Peroxidases/chemistry;Protein Conformation;Protein Denaturation;Protein Structure, Tertiary;Recombinant Proteins/chemistry;Spectrophotometry, Ultraviolet/methods;Synechocystis/metabolism;Thermodynamics;

Find related publications in this database (Keywords)
Two-domain structure
Gene duplication
Conformational stability
Thermal stability

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