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Gewählte Publikation:

Sohn, SB; Graf, AB; Kim, TY; Gasser, B; Maurer, M; Ferrer, P; Mattanovich, D; Lee, SY.
(2010): Genome-scale metabolic model of methylotrophic yeast Pichia pastoris and its use for in silico analysis of heterologous protein production
BIOTECHNOL J. 2010; 5(7): 705-715. FullText FullText_BOKU

Abstract:
The methylotrophic yeast Pichia pastoris has gained much attention during the last decade as a platform for producing heterologous recombinant proteins of pharmaceutical importance, due to its ability to reproduce post-translational modification similar to higher eukaryotes. With the recent release of the full genome sequence for P. pastoris, in-depth study of its functions has become feasible. Here we present the first reconstruction of the genome-scale metabolic model of the eukaryote P. pastoris type strain DSMZ 70382, PpaMBEL1254, consisting of 1254 metabolic reactions and 1147 metabolites compartmentalized into eight different regions to represent organelles. Additionally, equations describing the production of two heterologous proteins, human serum albumin and human superoxide dismutase, were incorporated. The protein-producing model versions of PpaMBEL1254 were then analyzed to examine the impact on oxygen limitation on protein production.
Autor*innen der BOKU Wien:
Gasser Brigitte
Mattanovich Diethard
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Culture Media -
Databases, Protein -
Fermentation -
Humans -
Models, Biological -
Pichia - metabolism
Protein Engineering -
Protein Processing, Post-Translational -
Recombinant Proteins - biosynthesis
Saccharomyces cerevisiae - metabolism
Serum Albumin - biosynthesis
Superoxide Dismutase - biosynthesis

Find related publications in this database (Keywords)
Genome-scale metabolic model
Heterologous protein production
HSA
hSOD
Pichia pastoris


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