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Gewählte Publikation:

Kainz, B; Steiner, K; Sleytr, UB; Pum, D; Toca-Herrera, JL; .
(2010): Fluorescence energy transfer in the bi-fluorescent S-layer tandem fusion protein ECFP-SgsE-YFP.
J Struct Biol. 2010; 172(3):276-283 FullText FullText_BOKU

This work reports for the first time on the fabrication of a bi-functional Slayer tandem fusion protein which is able to self-assemble on solid supports without losing its functionality. Two variants of the green fluorescent protein (GFP) were genetically combined with a self-assembly system having the remarkable opportunity to interact with each other and act as functional nanopatterning biocoating. The S-layer protein SgsE of Geobacillus stearothermophilus NRS 2004/3a was fused with the cyan ECFP donor protein at the SgsE N-terminus and with the yellow YFP acceptor protein at the C-terminus. The fluorescence energy transfer was studied with spectrofluorimetry, confocal microscopy and flow cytometry, whilst protein self-assembly (on silicon dioxide particles) and structural investigations were carried out with atomic force microscopy (AFM). The fluorescence resonance energy transfer efficiency of reassembled SgsE tandem protein was 20.0 +/- 6.1% which is almost the same transfer efficiency shown in solution (19.6 +/- 0.1%). This work shows that bi-fluorescent Slayer fusion proteins self-assemble on silica particles retaining their fluorescent properties. (C) 2010 Elsevier Inc. All rights reserved.
Autor*innen der BOKU Wien:
Pum Dietmar
Sleytr Uwe B.
Toca-Herrera José Luis
Find related publications in this database (using NML MeSH Indexing)
Flow Cytometry;Fluorescence Resonance Energy Transfer/methods*;Green Fluorescent Proteins/genetics;Green Fluorescent Proteins/metabolism*;Luminescent Proteins/genetics;Luminescent Proteins/metabolism*;Membrane Glycoproteins/genetics;Membrane Glycoproteins/metabolism*;Microscopy, Atomic Force;Microscopy, Confocal;Spectrometry, Fluorescence;

Find related publications in this database (Keywords)
S-layer tandem fusion protein
Fluorescent proteins
Fluorescence resonance energy transfer

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