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Gewählte Publikation:

Schoberer, J; Runions, J; Steinkellner, H; Strasser, R; Hawes, C; Osterrieder, A.
(2010): Sequential Depletion and Acquisition of Proteins during Golgi Stack Disassembly and Reformation
TRAFFIC. 2010; 11(11): 1429-1444. FullText FullText_BOKU

Herein, we report the stepwise transport of multiple plant Golgi membrane markers during disassembly of the Golgi apparatus in tobacco leaf epidermal cells in response to the induced expression of the GTP-locked Sar1p or Brefeldin A (BFA), and reassembly on BFA washout. The distribution of fluorescent Golgi-resident N-glycan processing enzymes and matrix proteins (golgins) with specific cis-trans-Golgi sub-locations was followed by confocal microscopy during disassembly and reassembly. The first event during Golgi disassembly was the loss of trans-Golgi enzymes and golgins from Golgi membranes, followed by a sequential redistribution of medial and cis-Golgi enzymes into the endoplasmic reticulum (ER), whilst golgins were relocated to the ER or cytoplasm. This event was confirmed by fractionation and immuno-blotting. The sequential redistribution of Golgi components in a trans-cis sequence may highlight a novel retrograde trafficking pathway between the trans-Golgi and the ER in plants. Release of Golgi markers from the ER upon BFA washout occurred in the opposite sequence, with cis-matrix proteins labelling Golgi-like structures before cis/medial enzymes. Trans-enzyme location was preceded by trans-matrix proteins being recruited back to Golgi membranes. Our results show that Golgi disassembly and reassembly occur in a highly ordered fashion in plants.
Autor*innen der BOKU Wien:
Schoberer Jennifer
Steinkellner Herta
Strasser Richard

Find related publications in this database (Keywords)
Brefeldin A
Golgi apparatus
N-glycan processing enzymes
Sar1p GTPase

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