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Gewählte Publikation:

Sekot, G; Posch, G; Messner, P; Matejka, M; Rausch-Fan, X; Andrukhov, O; Schaffer, C.
(2011): Potential of the Tannerella forsythia S-layer to Delay the Immune Response
J DENT RES. 2011; 90(1): 109-114. FullText FullText_BOKU

Abstract:
The periodontal pathogen Tannerella forsythia possesses a glycosylated S-layer as an outermost cell decoration. While the S-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. In the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type T. forsythia and an S-layer-deficient mutant were investigated. The mRNA expression levels of the pro-inflammatory mediators IL-1 beta, TNF-alpha, and IL-8 were analyzed by qPCR, and the production of the corresponding cytokines was investigated by ELISA. The S-layer-deficient T. forsythia mutant induced significantly higher levels of proinflammatory mediators compared with wild-type T. forsythia, especially at the early phase of response. Analysis of these data suggests that the S-layer of T. forsythia is an important virulence factor that attenuates the host immune response to this pathogen by evading the bacterium's recognition by the innate immune system. Abbreviations: DMSO, dimethylsulfoxide; FBS, fetal bovine serum; GAPDH, glycerinaldehyde- 3-phosphate-dehydrogenase; HGFs, human gingival fibroblasts; LPS, lipopolysaccharide; MEM, minimal essential medium; MTT, 3,4,5-dimethylthiazol- 2-yl-2,5-diphenyl tetrazolium bromide; OD, optical density; PBS, phosphate-buffered saline; qPCR, quantitative polymerase chain-reaction; SD, standard deviation; Tannerella forsythia ATCC 43037, Tf wt; Tannerella forsythia ATCC 43037 S-layer mutant, Tf Delta tfsAB.
Autor*innen der BOKU Wien:
Messner Paul
Posch Gerald
Schäffer Christina

Find related publications in this database (Keywords)
Tannerella forsythia
S-layer
immunology
cytokine production
macrophages


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