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Gewählte Publikation:

Gregori, C; Bauer, B; Schwartz, C; Kren, A; Schüller, C; Kuchler, K.
(2007): A genetic screen identifies mutations in the yeast WAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation.
FEBS J. 2007; 274(12):3094-3107 FullText FullText_BOKU

Exposure of the yeast Saccharomyces cerevisiae to weak organic acids such as the food preservatives sorbate, benzoate and propionate leads to the pronounced induction of the plasma membrane ATP-binding cassette (ABC) transporter, Pdr12p. This protein mediates efflux of weak acid anions, which is essential for stress adaptation. Recently, we identified War1p as the dedicated transcriptional regulator required for PDR12 stress induction. Here, we report the results from a genetic screen that led to the isolation of two war1 alleles encoding mutant variants, War1-28p and War1-42p, which are unable to support cell growth in the presence of sorbate. DNA sequencing revealed that War1-28 encodes a truncated form of the transcriptional regulator, and War1-42 carries three clustered mutations near the C-terminal activation domain. Although War1-42 is expressed and properly localized in the nucleus, the War1-42p variant fails to bind the weak-acid-response elements in the PDR12 promoter, as shown by in vivo footprinting. Importantly, in contrast with wild-type War1p, War1-42p is also no longer phosphorylated upon weak-acid challenge, demonstrating that phosphorylation of War1p, its activation and DNA binding are tightly linked processes that are essential for adaptation to weak-acid stress.
Autor*innen der BOKU Wien:
Schüller Christoph
Find related publications in this database (using NML MeSH Indexing)
ATP-Binding Cassette Transporters - biosynthesis
Adaptation, Physiological -
Amino Acid Sequence -
Base Sequence -
DNA, Fungal - metabolism
Mutation -
Phosphorylation -
Promoter Regions, Genetic -
Protein Processing, Post-Translational -
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae Proteins - biosynthesis
Sorbic Acid - pharmacology
Transcription Factors - genetics

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