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Gewählte Publikation:

Gregori, C; Schüller, C; Roetzer, A; Schwarzmüller, T; Ammerer, G; Kuchler, K.
(2007): The high-osmolarity glycerol response pathway in the human fungal pathogen Candida glabrata strain ATCC 2001 lacks a signaling branch that operates in baker's yeast.
Eukaryot Cell. 2007; 6(9):1635-1645 FullText FullText_BOKU

The high-osmolarity glycerol (HOG) mitogen-activated protein (MAP) kinase pathway mediates adaptation to high-osmolarity stress in the yeast Saccharomyces cerevisiae. Here we investigate the function of HOG in the human opportunistic fungal pathogen Candida glabrata. C. glabrata sho1 Delta (Cgsho1 Delta) deletion strains from the sequenced ATCC 2001 strain display severe growth defects under hyperosmotic conditions, a phenotype not observed for yeast sho1 Delta mutants. However, deletion of CgSHO1 in other genetic backgrounds fails to cause osmostress hypersensitivity, whereas cells lacking the downstream MAP kinase Pbs2 remain osmosensitive. Notably, ATCC 2001 Cgsho1 Delta cells also display methylglyoxal hypersensitivity, implying the inactivity of the Sln1 branch in ATCC 2001. Genomic sequencing of CgSSK2 in different C. glabrata backgrounds demonstrates that ATCC 2001 harbors a truncated and mutated Cgssk2-1 allele, the only orthologue of yeast SSK2/SSK22 genes. Thus, the osmophenotype of ATCC 2001 is caused by a point mutation in Cgssk2-1, which debilitates the second HOG pathway branch. Functional complementation experiments unequivocally demonstrate that HOG signaling in yeast and C. glabrata share similar functions in osmostress adaptation. In contrast to yeast, however, Cgshol Delta mutants display hypersensitivity to weak organic acids such as sorbate and benzoate. Hence, Cgshol is also implicated in modulating weak acid tolerance, suggesting that HOG signaling in C. glabrata mediates the response to multiple stress conditions.
Autor*innen der BOKU Wien:
Schüller Christoph
Find related publications in this database (using NML MeSH Indexing)
Acids - pharmacology
Amino Acid Sequence -
Candida glabrata - drug effects
Fungal Proteins - genetics
Gene Deletion -
Genome, Fungal - genetics
Glycerol - metabolism
Hot Temperature -
Humans -
MAP Kinase Kinase Kinases - genetics
Membrane Proteins - genetics
Molecular Sequence Data -
Osmolar Concentration -
Point Mutation -
Saccharomyces cerevisiae - metabolism
Signal Transduction - genetics

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