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Gewählte Publikation:

Naetar, N; Hutter, S; Dorner, D; Dechat, T; Korbei, B; Gotzmann, J; Beug, H; Foisner, R.
(2007): LAP2alpha-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit.
J Cell Sci. 2007; 120(Pt 5):737-747 FullText FullText_BOKU

Abstract:
Lamina-associated polypeptide 2 alpha (LAP2 alpha) is a nuclear protein dynamically associating with chromatin during the cell cycle. In addition, LAP2 alpha interacts with A-type lamins and retinoblastoma protein and regulates cell cycle progression via the E2F-Rb pathway. Using yeast two-hybrid analysis and three independent in vitro binding assays we identified a new LAP2 alpha interaction partner of hitherto unknown functions, which we termed LINT-25. LNT-25 protein levels were upregulated during G1 phase in proliferating cells and upon cell cycle exit in quiescence, senescence and differentiation. Upon cell cycle exit LINT-25 accumulated in heterochromatin foci, and LAP2 alpha protein levels were downregulated by proteasomal degradation. Although LAP2 alpha was not required for the upregulation and reorganization of LINT-25 during cell cycle exit, transient expression of LINT-25 in proliferating cells caused loss of LAP2 alpha and subsequent cell death. Our data show a role of LINT-25 and LAP2 alpha during cell cycle exit, in which LINT-25 acts upstream of LAP2 alpha.
Autor*innen der BOKU Wien:
Korbei Barbara
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals -
Cell Cycle - genetics
Cell Cycle Proteins - genetics
Cell Differentiation - drug effects
Cell Line -
Cells, Cultured -
Chromatin - metabolism
Chromosomal Proteins, Non-Histone - genetics
DNA-Binding Proteins - genetics
Gene Expression Regulation -
HeLa Cells -
Humans -
Immunoblotting -
Immunoprecipitation -
Lamins - metabolism
Membrane Proteins - genetics
Mice -
Microscopy, Fluorescence -
Nuclear Proteins - genetics
Protein Binding -
Reverse Transcriptase Polymerase Chain Reaction -
Transfection -
Two-Hybrid System Techniques -



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