BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Kökpinar, O; Harkensee, D; Kasper, C; Scheper, T; Zeidler, R; Reif, OW; Ulber, R.
(2006): Innovative modular membrane adsorber system for high-throughput downstream screening for protein purification.
Biotechnol Prog. 2006; 22(4):1215-1219 FullText FullText_BOKU

Abstract:
To develop the most efficient strategy for the purification of proteins, two types of adsorber membrane devices with different functionalities were designed and tested: 8-strips and single spin columns. The most suitable type of membrane adsorber and the optimal chromatographic loading/elution conditions for several target proteins from different biological matrices could be determined simultaneously in microliter scale. Ion exchange (IEX), metal chelate (MC), and Concanavalin A (Con A) modified membrane types were tested in the devices. Bovine serum albumin (BSA) and lysozyme were used as model proteins for investigations of the binding capacity and protein recovery percentage of the 8-strip anion exchange and the cation exchange membrane. The isolation of His(6)-tagged proteins, Bgl-His and GFP-His from fermentation broth and lysate, respectively, was performed using an 8-strip metal chelate affinity membrane loaded with different metal ions. Separation behavior of a ternary protein mixture (BSA, lysozyme, and Bgl-His) was studied in 8-strips IEX and metal chelate membrane chromatography. The Con A affinity devices were developed on the basis of metal chelate membrane spin columns loaded with Cu2+ ions and investigated using glucose oxidase (GOD) as model protein. In summary, the advantages of the membrane adsorber technology, such as fast processing and easy scale-up, were utilized. The devices made it possible to load the membrane directly with preclarified fermentation broth or cell lysate and separate the protein of interest often in a single step.
Autor*innen der BOKU Wien:
Almeria Ciarra
Kasper Cornelia
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Adsorption -
Chelating Agents - chemistry
Concanavalin A - chemistry
Glucose Oxidase - isolation & purification
Ion Exchange -
Membranes, Artificial -
Molecular Weight -
Muramidase - isolation & purification
Serum Albumin, Bovine - isolation & purification
Surface Properties -



Altmetric:
© BOKU Wien Impressum