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Gewählte Publikation:

Garcs, G; Effenberger, M; Najdrowski, M; Wackwitz, C; Gronauer, A; Wilderer, PA; Lebuhn, M.
(2006): Quantification of Cryptosporidium parvum in anaerobic digesters treating manure by (reverse-transcription) quantitative real-time PCR, infectivity and excystation tests.
Water Sci Technol. 2006; 53(8):195-202 FullText FullText_BOKU

Abstract:
The survival of Cryptosporidium parvum oocysts in anaerobic digesters treating manure was investigated for mesophilic, thermophilic, and a combined treatment (mesophilic-thermophilic-mesophilic) under different retention times of oocysts in the reactors. C. parvum DNA was extracted with an optimised protocol, and its amount determined by quantitative real-time PCR (qPCR). Results indicated noteworthy differences in DNA content after the different treatments. DNA was not degraded during the process. However, excystation and infectivity tests showed a reduction of viable oocyst numbers of >= 2 and >= 5 log units after the thermophilic treatment in two different experiments. Thus qPCR-targeting DNA can overestimate the number of oocysts that survive and remain viable after anaerobic digestion. However, targeting DNA is suitable to indicate the presence or absence of oocysts. Reverse transcription qPCR (RT-qPCR) targeting C. parvum hsp70 mRNA successfully indicated the presence of viable fraction of oocysts.
Autor*innen der BOKU Wien:
Gronauer Andreas
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Anaerobiosis -
Animals -
Bacteria, Anaerobic - metabolism
Bioreactors -
Cryptosporidium parvum - genetics
DNA, Protozoan - analysis
HSP70 Heat-Shock Proteins - genetics
Manure - microbiology
Oocysts - cytology
RNA, Messenger - analysis
Reverse Transcriptase Polymerase Chain Reaction -

Find related publications in this database (Keywords)
Cryptosporidium parvum
DNA
hygiene
manure
mRNA
quantitative real-time PCR


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