BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Staudacher, E; Altmann, F; Glössl, J; März, L; Schachter, H; Kamerling, JP; Hård, K; Vliegenthart, JF.
(1991):
GDP-fucose: beta-N-acetylglucosamine (Fuc to (Fuc alpha 1----6GlcNAc)-Asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.
Eur J Biochem. 1991; 199(3):745-751
FullText
FullText_BOKU
- Abstract:
- Incubation of honeybee (Apis mellifica) venom-gland extracts with GDP-[C-14]fucose and GlcNAc-beta-1 --> 2Man-alpha-1 --> 6(GlcNAc-beta-1 --> 2Man-alpha-1 --> 3)Man-beta-1 --> 4GlcNAc-beta-1 --> 4(Fuc-alpha-1 --> 6)GlcNAc-beta-1 --> N-Asn-peptide(NAc) gave a labeled product in 40% yield. Analysis by 500-MHz H-1-NMR spectroscopy indicated the transferred fucose (Fuc) residue to be alpha-1 --> 3-linked to the Asn-bound GlcNAc. Further proof was provided by one-dimensional and two-dimensional H-1-NMR analysis of the incubation mixture, after incubation with beta-N-acetylhexosaminidase. The established carbohydrate structure [GRAPHICS] proves the existence of a novel alpha-1 --> 3-fucosyltransferase with the ability to effect difucosylation of the Asn-bound GlcNAc in N-glycans.
- Autor*innen der BOKU Wien:
- Altmann Friedrich
- Glößl Josef
- März Leopold
- Staudacher Erika
- Find related publications in this database (using NML MeSH Indexing)
- Acetylglucosamine - metabolism
- Animals -
- Bee Venoms -
- Bees - enzymology
- Carbohydrate Conformation -
- Carbohydrate Sequence -
- Fucosyltransferases - isolation & purification
- Guanosine Diphosphate Fucose - metabolism
- Kinetics -
- Magnetic Resonance Spectroscopy - methods
- Molecular Sequence Data -
- Substrate Specificity -
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