BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Couto, SR; Sanroman, MA; Hofer, D; Gubitz, GM.
(2004):
Stainless steel sponge: a novel carrier for the immobilisation of the white-rot fungus Trametes hirsuta for decolourization of textile dyes
BIORESOURCE TECHNOL. 2004; 95(1): 67-72.
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- Abstract:
- Alginate beads.. polyurethane foam, nylon sponge and stainless steel sponge were tested as carrier materials for the white-rot fungus Trametes hirsuta for laccase production under submerged fermentation conditions. Stainless steel sponge was the best carrier material leading to the highest laccase activities of up to 800 U/l after 8 days of cultivation. These values are higher than those reported to date operating with inert supports and without inducer addition. In a 1-1 bioreactor containing T hirsuta immobilised on stainless steel sponge laccase activities of about 2200 U/l were obtained when the culture medium was supplemented with I mM copper sulphate. There were no operational problems with this system during culturing time. The textile dye Indigo Carmine was almost totally degraded in 3 days by T hirsuta grown in this bioreactor, while Lanaset Marine was degraded in two successive batches, reaching in the first batch a decolourization percentage of about 82% in 15 h and in the second one by 71% in 28 h. Results obtained after inhibition of growth of T hirsuta by antibiotics indicated that dye decolourization could not exclusively be attributed to laccase activity. (C) 2004 Elsevier Ltd. All rights reserved.
- Autor*innen der BOKU Wien:
- Gübitz Georg
- Find related publications in this database (Keywords)
- bioreactor
- decolourization
- laccase
- stainless steel sponge
- submerged cultivation
- textile dyes
- Trametes hirsuta
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