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Gewählte Publikation:

Walther, C; Mayer, S; Trefilov, A; Sekot, G; Hahn, R; Jungbauer, A; Dürauer, A.
(2014): Prediction of inclusion body solubilization from shaken to stirred reactors.
Biotechnol Bioeng. 2014; 111(1):84-94 FullText FullText_BOKU

Abstract:
Inclusion bodies (IBs) were solubilized in a mu-scale system using shaking microtiter plates or a stirred tank reactor in a laboratory setting. Characteristic dimensionless numbers for mixing, the Phase number Ph and Reynolds number Re did not correlate with the kinetics and equilibrium of protein solubilization. The solubilization kinetics was independent of the mixing system, stirring or shaking rate, shaking diameter, and energy input. Good agreement was observed between the solubilization kinetics and yield on the mu-scale and laboratory setting. We show that the IB solubilization process is controlled predominantly by pore diffusion. Thus, for the process it is sufficient to keep the IBs homogeneously suspended, and additional power input will not improve the process. The high-throughput system developed on the mu-scale can predict solubilization in stirred reactors up to a factor of 500 and can therefore be used to determine optimal solubilization conditions on laboratory and industrial scale. Biotechnol. Bioeng. 2014;111: 84-94. (c) 2013 Wiley Periodicals, Inc.
Autor*innen der BOKU Wien:
Dürauer Astrid
Hahn Rainer
Jungbauer Alois
Mayer Sabrina
Walther Cornelia
BOKU Gendermonitor:


Find related publications in this database (Keywords)
E
coli
solubilization
scale up
mixing techniques
stirred tank reactor
microtiter plate


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