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Gewählte Publikation:

Rocasalbas, G; Francesko, A; Tourino, S; Fernandez-Francos, X; Guebitz, GM; Tzanov, T.
(2013): Laccase-assisted formation of bioactive chitosan/gelatin hydrogel stabilized with plant polyphenols
CARBOHYD POLYM. 2013; 92(2): 989-996. FullText FullText_BOKU

Abstract:
Laccase-assisted simultaneous cross-linking and functionalization of chitosan/gelatin blends with phenolic compounds from Hamamelis virginiana was investigated for the development of bioactive hydrogel dressings. The potential of these hydrogels for chronic wound treatment was evaluated in vitro, assessing their antibacterial and inhibitory effect on myeloperoxidase and collagenase. Rheological studies revealed that the mechanical properties of the hydrogels were a function of the enzymatic reaction time. Stable hydrogels and resistant to lysozyme degradation were achieved after 2 h laccase reaction. The inhibitory capacity of the hydrogel for myeloperoxidase and collagenase was 32% and 79% respectively after 24 h incubation. Collagenase activity was additionally suppressed by adsorption (20%) of the enzyme onto the hydrogel. Therefore, the bioactive properties of the hydrogels were due to the effect of both released phenolic compounds and the permanently functionalized platform itself. The hydrogels showed antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus. (C) 2012 Elsevier Ltd. All rights reserved.
Autor*innen der BOKU Wien:
Gübitz Georg
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Chitosan
Chronic wounds
Gelatin
Laccase
Hydrogels
Phenolic compounds


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