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Gewählte Publikation:

Marzban, G; Kinaciyan, T; Maghuly, F; Brunner, R; Gruber, C; Hahn, R; Jensen-Jarolim, E; Laimer, M; .
(2014): Impact of sulfur and vitamin C on the allergenicity of Mal d 2 from apple (Malus domestica).
J Agric Food Chem. 2014; 62(30):7622-7630 FullText FullText_BOKU

Mal d 2 is a minor allergen from apple which shows a high conformational stability due to its eight conserved disulfide bridges. Chemical reduction of disulfide bridges and linearization of Mal d 2 lead to enhanced IgE reactivity in vitro and indicate a higher potential for allergenicity. Since food preservatives such as sulfur and vitamin C are reducing and denaturing agents, their influence on Mal d 2 allergenicity was verified by simulated food processing conditions. The immunoreactivity of purified Mal d 2 was investigated after different treatments in vitro and in vivo using IgG/IgE Western blotting, mediator-releasing cell assay, and skin prick and oral smear tests. The conformational changes of Mal d 2 upon addition of 1% and 5% vitamin C were also monitored by attenuated total reflectance Fourier transform infrared spectroscopy. The results show no positive skin and oral smear test reactivity to native, heated, or vitamin C-treated purified Mal d 2. Furthermore, the results confirm that sulfur in combination with heat treatment can influence the structural integrity and thus the allergenicity of Mal d 2, while vitamin C is too weak as a reducing agent to change allergenicity.
Autor*innen der BOKU Wien:
Grünwald-Gruber Clemens
Hahn Rainer
Laimer Margit
Maghuly Fatemeh
Marzban Gorji
Find related publications in this database (using NML MeSH Indexing)
Allergens/immunology*;Allergens/isolation & purification;Animals;Antigens, Plant/immunology*;Antigens, Plant/isolation & purification;Ascorbic Acid/pharmacology*;Cell Line, Tumor;Electrophoresis, Polyacrylamide Gel;Food Handling;Food Hypersensitivity/immunology;Food Hypersensitivity/prevention & control;Hot Temperature;Humans;Immunoglobulin E/immunology;Malus/immunology*;Plant Proteins/chemistry;Plant Proteins/immunology;Rats;Skin Tests/methods;Spectroscopy, Fourier Transform Infrared;Sulfur/pharmacology*;Transfection;beta-N-Acetylhexosaminidases/metabolism;

Find related publications in this database (Keywords)
thaumatin-like proteins
pathogenesis-related proteins
food safety
IgE reactivity
oral allergy syndrome

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