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Gewählte Publikation:

Gonzalez, J; Lopez, G; Argueta, S; Escalera-Fanjul, X; el Hafidi, M; Campero-Basaldua, C; Strauss, J; Riego-Ruiz, L; Gonzalez, A.
(2017): Diversification of Transcriptional Regulation Determines Subfunctionalization of Paralogous Branched Chain Aminotransferases in the Yeast Saccharomyces cerevisiae
GENETICS. 2017; 207(3): 975-991. FullText FullText_BOKU

Saccharomyces cerevisiae harbors BAT1 and BAT2 paralogous genes that encode branched chain aminotransferases and have opposed expression profiles and physiological roles. Accordingly, in primary nitrogen sources such as glutamine, BAT1 expression is induced, supporting Bat1-dependent valine-isoleucine-leucine (VIL) biosynthesis, while BAT2 expression is repressed. Conversely, in the presence of VIL as the sole nitrogen source, BAT1 expression is hindered while that of BAT2 is activated, resulting in Bat2-dependent VIL catabolism. The presented results confirm that BAT1 expression is determined by transcriptional activation through the action of the Leu3-alpha-isopropylmalate (alpha-IPM) active isoform, and uncovers the existence of a novel alpha-IPM biosynthetic pathway operating in a put3 Delta mutant grown on VIL, through Bat2-Leu2-Leu1 consecutive action. The classic alpha-IPM biosynthetic route operates in glutamine through the action of the leucine-sensitive alpha-IPM synthases. The presented results also show that BAT2 repression in glutamine can be alleviated in a ure2 Delta mutant or through Gcn4-dependent transcriptional activation. Thus, when S. cerevisiae is grown on glutamine, VIL biosynthesis is predominant and is preferentially achieved through BAT1; while on VIL as the sole nitrogen source, catabolism prevails and is mainly afforded by BAT2.
Autor/innen der BOKU Wien:
Strauss Joseph
BOKU Gendermonitor:

Find related publications in this database (Keywords)
functional diversification
expression regulation
amino acid metabolism
paralogous genes

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