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Gewählte Publikation:

Vastano, M; Pellis, A; Immirzi, B; Dal Poggetto, G; Malinconico, M; Sannia, G; Guebitz, GM; Pezzella, C.
(2017): Enzymatic production of clickable and PEGylated recombinant polyhydroxyalkanoates
GREEN CHEM. 2017; 19(22): 5494-5504. FullText FullText_BOKU

Abstract:
Polyhydroxyalkanoates (PHAs) are microbial polyesters attracting great attention while further functionalisation could widen their applicability. Various Escherichia coli based production systems using the PHA biosynthetic operon from Bacillus cereus 6E/2 were designed to enhance the accumulation of PHAs with high medium chain length moieties. Media optimization and system engineering were applied, yielding the production of PHAs up to 260 mg L-1. Polymer characterization revealed a low grade of crystallinity and remarkable hydrophobic features. For further functionalization, a novel enzyme based strategy was developed. Lipase B from Candida antarctica (CaLB) was used to catalyze the terminal coupling of PHAs with: (i) dimethyl itaconate (DMI) in order to introduce reactive side chain vinyl moieties for the easy coupling of functional molecules and/or (ii) biocompatible polyethylene glycol (PEG) to tune polymer hydrophilicity. The functionalized DMI-PHA, PEG-PHA and PEG-DMI-PHA polymers obtained and characterised by NMR, GPC, FT-IR, and WCA in this study open new perspectives on the use of PHAs as biodegradable and biocompatible materials of choice for biomedical applications.
Autor/innen der BOKU Wien:
Gübitz Georg
Pellis Alessandro
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