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Gewählte Publikation:

Kittibunchakul, S; van Leeuwen, SS; Dijkhuizen, L; Haltrich, D; Nguyen TH.
(2020): Structural Comparison of Different Galacto-oligosaccharide Mixtures Formed by beta-Galactosidases from Lactic Acid Bacteria and Bifidobacteria
J AGR FOOD CHEM. 2020; 68(15): 4437-4446. FullText FullText_BOKU

The LacLM-type beta-galactosidase from Lactobacillus helveticus DSM 20075 expressed in both Escherichia coli (EcoliBL21Lh beta-gal) and Lactobacillus plantarum (Lp609Lh beta-gal) was tested for their potential to form galacto-oligosaccharides (GOS) from lactose. The Lh-GOS mixture formed by beta-galactosidase from L. helveticus, together with three GOS mixtures produced using beta-galactosidases of both the LacLM and the LacZ type from other lactic acid bacteria, namely, L. reuteri (Lr-GOS), L. bulgaricus (Lb-GOS), and Streptococcus thermophilus (St-GOS), as well as two GOS mixtures (Br-GOS1 and Br-GOS2) produced using beta-galactosidases (beta-gal I and beta-gal II) from Bifidobacterium breve, was analyzed and structurally compared with commercial GOS mixtures analyzed in previous work (Vivinal GOS, GOS I, GOS III, and GOS V) using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), high-performance size-exclusion chromatography with a refractive index (RI) detector (HPSEC-RI), and one-dimensional NMR spectroscopy. beta-Galactosidases from lactic acid bacteria and B. breve displayed a preference to form beta-(1 -> 6)- and beta-(1 -> 3)-linked GOS. The GOS mixtures produced by these enzymes consisted of mainly DP2 and DP3 oligosaccharides, accounting for similar to 90% of all GOS components. GOS mixtures obtained with beta-galactosidases from lactic acid bacteria and B. breve were quite similar to the commercial GOS III mixture in terms of product spectrum and showed a broader product spectrum than the commercial GOS V mixture. These GOS mixtures also contained a number of GOS components that were absent in the commercial Vivinal GOS (V-GOS).
Autor/innen der BOKU Wien:
Haltrich Dietmar
Nguyen Thu Ha
BOKU Gendermonitor:

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lactic acid bacteria

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