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Gewählte Publikation:

Sun, PC; Valenzuela, SV; Chunkrua, P; Pastor, FIJ; Laurent, CVFP; Ludwig, R; Van Berkel, WJH; Kabel, MA.
(2021): Oxidized Product Profiles of AA9 Lytic Polysaccharide Monooxygenases Depend on the Type of Cellulose
ACS SUSTAIN CHEM ENG. 2021; 9(42): 14124-14133. FullText FullText_BOKU

Lytic polysaccharide monooxygenases (LPMOs) are essential for enzymatic conversion of lignocellulose-rich biomass in the context of biofuels and platform chemicals production. Considerable insight into the mode of action of LPMOs has been obtained, but research on the cellulose specificity of these enzymes is still limited. Hence, we studied the product profiles of four fungal Auxiliary Activity family 9 (AA9) LPMOs during their oxidative cleavage of three types of cellulose: bacterial cellulose (BC), Avicel PH-101 (AVI), and regenerated amorphous cellulose (RAC). We observed that attachment of a carbohydratebinding module 1 (CBM1) did not change the substrate specificity of LPMO9B from Myceliophthora thermophila C1 (MtLPMO9B) but stimulated the degradation of all three types of cellulose. A detailed quantification of oxidized ends in both soluble and insoluble fractions, as well as characterization of oxidized cellooligosaccharide patterns, suggested that MtLPMO9B generates mainly oxidized cellobiose from BC, while producing oxidized cellooligosaccharides from AVI and RAC ranged more randomly from DP2-8. Comparable product profiles, resulting from BC, AVI, and RAC oxidation, were found for three other AA9 LPMOs. These distinct cleavage profiles highlight cellulose specificity rather than an LPMO-dependent mechanism and may further reflect that the product profiles of AA9 LPMOs are modulated by different cellulose types.
Autor*innen der BOKU Wien:
Ludwig Roland
BOKU Gendermonitor:

Find related publications in this database (Keywords)
Auxiliary Activity (AA)
Lytic polysaccharide monooxygenase (LPMO)
Carbohydrate-binding module (CBM)
Oxidized cello-oligosaccharide
Product profile

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