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Gewählte Publikation:

Snella, B; Grain, B; Vicogne, J; Capet, F; Wiltschi, B; Melnyk, O; Agouridas, V.
(2022): Fast Protein Modification in the Nanomolar Concentration Range Using an Oxalyl Amide as Latent Thioester
ANGEW CHEM INT EDIT. 2022; 61(29), e202204992 FullText FullText_BOKU

Abstract:
We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on demand to enable ligation reactions with peptide concentrations as low as a few hundred nM at rates approaching 30 M-1 s(-1).
Autor*innen der BOKU Wien:
Wiltschi Birgit
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Latency
N
S-Acyl Shift Systems
Non-Native Ligation
Protein Modification
Thioester


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