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Gewählte Publikation:

Zemkollari, M; Blaukopf, M; Grabherr, R; Staudacher, E.
(2023): Expression and Characterisation of the First Snail-Derived UDP-Gal: Glycoprotein-N-acetylgalactosamine beta-1,3-Galactosyltransferase (T-Synthase) from Biomphalaria glabrata
MOLECULES. 2023; 28(2), 552 FullText FullText_BOKU

UDP-Gal: glycoprotein-N-acetylgalactosamine beta-1,3-galactosyltransferase (T-synthase, EC catalyses the transfer of the monosaccharide galactose from UDP-Gal to GalNAc-Ser/Thr, synthesizing the core 1 mucin type O-glycan. Such glycans play important biological roles in a number of recognition processes. The crucial role of these glycans is acknowledged for mammals, but a lot remains unknown regarding invertebrate and especially mollusc O-glycosylation. Although core O-glycans have been found in snails, no core 1 beta-1,3-galactosyltransferase has been described so far. Here, the sequence of the enzyme was identified by a BlastP search of the NCBI Biomphalaria glabrata database using the human T-synthase sequence (NP_064541.1) as a template. The obtained gene codes for a 388 amino acids long transmembrane protein with two putative N-glycosylation sites. The coding sequence was synthesised and expressed in Sf9 cells. The expression product of the putative enzyme displayed core 1 beta-1,3-galactosyltransferase activity using pNP-alpha-GalNAc as the substrate. The enzyme showed some sequence homology (49.40% with Homo sapiens, 53.69% with Drosophila melanogaster and 49.14% with Caenorhabditis elegans) and similar biochemical parameters with previously characterized T-synthases from other phyla. In this study we present the identification, expression and characterisation of the UDP-Gal: glycoprotein-N-acetylgalactosamine beta-1,3-galactosyltransferase from the fresh-water snail Biomphalaria glabrata, which is the first cloned T-synthase from mollusc origin.
Autor*innen der BOKU Wien:
Blaukopf Markus
Grabherr Reingard
Staudacher Erika
Zemkollari Marilica

Find related publications in this database (Keywords)
Biomphalaria glabrata

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