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Gewählte Publikation:

Dmytruk, KV; Ruchala, J; Fayura, LR; Chrzanowski, G; Dmytruk, OV; Tsyrulnyk, AO; Andreieva, YA; Fedorovych, DV; Motyka, OI; Mattanovich, D; Marx, H; Sibirny, AA.
(2023): Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeasts
MICROB CELL FACT. 2023; 22(1), 132 FullText FullText_BOKU

BackgroundActinomycetes Streptomyces davaonensis and Streptomyces cinnabarinus synthesize a promising broad-spectrum antibiotic roseoflavin, with its synthesis starting from flavin mononucleotide and proceeding through an immediate precursor, aminoriboflavin, that also has antibiotic properties. Roseoflavin accumulation by the natural producers is rather low, whereas aminoriboflavin accumulation is negligible. Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known.ResultsRoseoflavin biosynthesis genes have been expressed in riboflavin- or FMN-overproducing yeast strains of Candida famata and Komagataella phaffii. Both these strains accumulated aminoriboflavin, whereas only the latter produced roseoflavin. Aminoriboflavin isolated from the culture liquid of C. famata strain inhibited the growth of Staphylococcus aureus (including MRSA) and Listeria monocytogenes. Maximal accumulation of aminoriboflavin in shake-flasks reached 1.5 mg L- 1 (C. famata), and that of roseoflavin was 5 mg L- 1 (K. phaffii). Accumulation of aminoriboflavin and roseoflavin by K. phaffii recombinant strain in a bioreactor reached 22 and 130 mg L- 1, respectively. For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all.ConclusionsYeast recombinant producers of bacterial antibiotics aminoriboflavin and roseoflavin were constructed and evaluated.
Autor*innen der BOKU Wien:
Marx Hans
Mattanovich Diethard

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Metabolic engineering

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