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Gewählte Publikation:

Karner, L; Drechsler, S; Metzger, M; Slezak, P; Zipperle, J; Pinar, G; Sterflinger, K; Leisch, F; Grillari, J; Osuchowski, M; Dungel, P; .
(2020): Contamination of wounds with fecal bacteria in immuno-suppressed mice.
Sci Rep. 2020; 10(1):11494 FullText FullText_BOKU

Abstract:
Immunocompromised patients are predisposed to chronically infected wounds. Especially ulcers in the dorsal region often experience secondary polymicrobial infections. However, current wound infection models mostly use single-strain bacteria. To mimic clinically occurring infections caused by fecal contamination in immunocompromised/immobile patients, which differ significantly from single-strain infections, the present study aimed at the establishment of a new mouse model using infection by fecal bacteria. Dorsal circular excision wounds in immunosuppressed mice were infected with fecal slurry solution in several dilutions up to 1:8,000. Impact of immunosuppressor, bacterial load and timing on development of wound infections was investigated. Wounds were analyzed by scoring, 3D imaging and swab analyses. Autofluorescence imaging was not successful. Dose-finding of cyclophosphamide-induced immunosuppression was necessary for establishment of bacterial wound infections. Infection with fecal slurry diluted 1:166 to 1:400 induced significantly delayed wound healing (p < 0.05) without systemic reactions. Swab analyses post-infection matched the initial polymicrobial suspension. The customized wound score confirmed significant differences between the groups (p < 0.05). Here we report the establishment of a simple, new mouse model for clinically occurring wound infections by fecal bacteria and the evaluation of appropriate wound analysis methods. In the future, this model will provide a suitable tool for the investigation of complex microbiological interactions and evaluation of new therapeutic approaches.
Autor/innen der BOKU Wien:
Grillari Johannes
Leisch Friedrich
Pinar Guadalupe
Sterflinger-Gleixner Katja
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