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Gewählte Publikation:

Zand, E; Schottroff, F; Steinacker, E; Mae-Gano, J; Schoenher, C; Wimberger, T; Wassermann, KJ; Jaeger, H.
(2021): Advantages and limitations of various treatment chamber designs for reversible and irreversible electroporation in life sciences
BIOELECTROCHEMISTRY. 2021; 141, 107841 FullText FullText_BOKU

Abstract:
The fundamental mechanisms of pulsed electric fields on biological cells are not yet fully elucidated, though it is apparent that membrane electroporation plays a crucial role. Little is known about treatment-chamber-specific effects, and systematic studies are scarce. Thus, the present study evaluates the (dis-)advantages of various treatment chamber designs for liquid applications at differing scales. Three chambers, namely parallel plate microfluidic (V: 0.1 ml/min; titanium electrodes), co-linear meso (V: 5.0 ml/min; stainless steel electrodes), and co-linear macro (V: 83.3 ml/min; stainless steel electrodes) chambers, were studied. Electroporation effects on Escherichia coli in media with 0.1-10.0 mS/cm were evaluated by plate counts and flow cytometry at 8, 16, and 20 kV/cm. For the microfluidic chamber, predominantly irreversible electroporation (2.5 logs(10) reductions) was seen at 0.1 mS/cm, while high irreversible electroporation (4.2 logs(10) reductions) at 10.0 mS/cm was observed for the macro chamber. The meso chamber indicated a similar trend towards increased conductivity, even though only low inactivation levels were present. Variation in conductivity and electrode configuration or area likely induces effects resulting in distinct electroporation levels, as observed for the micro and macro chamber. Suitable application scenarios, depending on targeted electroporation effects, were suggested. (C) 2021 The Author(s). Published by Elsevier B.V.
Autor*innen der BOKU Wien:
Jäger Henry
Schönher Christoph
Schottroff Felix
Zand Elena
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Pulsed electric fields
Microfluidic
Treatment chamber design
Electrical conductivity
Reversible and irreversible electroporation
Escherichia coli


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