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Gewählte Publikation:

Braunschmid, V; Binder, K; Fuerst, S; Subagia, R; Danner, C; Weber, H; Schwaiger, N; Nyanhongo, GS; Ribitsch, D; Guebitz, GM.
(2021): Comparison of a fungal and a bacterial laccase for lignosulfonate polymerization
PROCESS BIOCHEM. 2021; 109: 207-213. FullText FullText_BOKU

Lignin is the second most abundant biopolymer on earth and accrues in large amounts in the pulp-and paper industry. While currently lignins are mainly used for low-value applications such as energy production, recently the potential of laccases for upgrading lignins has been demonstrated. In this study, two laccases of fungal and bacterial origin were characterized regarding their potential to polymerize lignosulfonate. The laccases MaL1 from Melanocarpus albomyces, and SrLA from Streptomyces rochei were heterologously expressed and showed typical characteristics of laccases, like acidic pH optima for ABTS at pH 4 and 5, respectively, and temperature optima at 50 degrees C and 80 degrees C. Polymerization of lignosulfonate with MaL1 led to an almost two-fold increase of the molecular weight, according to size exclusion (SEC) multiangle laser light scattering (MALLS) analysis. In contrast, SrLA showed considerably less activity on lignosulfonates, as measured based on oxygen consumption and SEC-MALLS.
Autor*innen der BOKU Wien:
Braunschmid Verena
Gübitz Georg
Nyanhongo Gibson Stephen
Ribitsch Doris
Subagia Raditya
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Lignosulfonate polymerization

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