University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal

Logo BOKU Resarch Portal

Selected Publication:

Toca-Herrera, JL; Küpcü, S; Diederichs, V; Moncayo, G; Pum, D; Sleytr, UB.
(2006): Fluorescence emission properties of S-Layer enhanced green fluorescent fusion protein as a function of temperature, pH conditions, and guanidine hydrochloride concentration.
Biomacromolecules. 2006; 7(12):3298-3301 FullText FullText_BOKU

Abstract:
The fluorescent properties of the S-layer enhanced green fluorescent fusion protein (rSbpA(31-1068)/EGFP) were investigated as a function of temperature, pH conditions, and guanidine hydrochloride concentration. These results were compared to the fluorescent properties of the recombinant enhanced green fluorescent protein (EGFP) and an equimolar mixture of the S-layer protein rSbpA and EGFP. The intensity of the fluorescence emission of the EGFP at 510 nm, after excitation at 490 nm, is not affected by the presence of rSbpA, either as a fusion partner or as a free protein in solution. In each of the three protein systems, the emission intensity at 510 nm reaches its maximum value between pH 7 and 9 at 20 degrees C and at 0 M guanidine hydrochloride. No fluorescence could be measured at pH 4 and 6 M guanidine hydrochloride. These results show that the S-layer fusion protein (rSbpA31-1068/EGFP) is a suitable candidate for future applications in nanobiotechonology at a wide range of pH, temperature, and guanidine hydrochloride concentrations.
Authors BOKU Wien:
Küpcü Seta
Pum Dietmar
Sleytr Uwe B.
Toca-Herrera José Luis
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Green Fluorescent Proteins - analysis
Guanidine -
Hydrogen-Ion Concentration -
Recombinant Fusion Proteins - analysis
Spectrometry, Fluorescence - methods
Thermodynamics -



Altmetric:
© BOKU Wien Imprint