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Selected Publication:
Schindler, S; Missbichler, B; Walther, C; Sponring, M; Cserjan-Puschmann, M; Auer, B; Schneider, R; Durauer, A.
(2016):
N-pro fusion technology: On-column complementation to improve efficiency in biopharmaceutical production
PROTEIN EXPRES PURIF. 2016; 120: 42-50.
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- Abstract:
- N-pro fusion technology, a highly efficient system for overexpression of proteins and peptides in Escherichia coli, was further developed by splitting the autoprotease N-pro into two fragments to generate a functional complementation system. The size of the expression tag is thus reduced from 168 to 58 amino acids, so by 66%. Upon complementation of the fragments auto-proteolytic activity is restored. This process has been shown for three model proteins of different size, a short 16 aa-peptide, MCP-1, and lysozyme. Moreover, the complementation was still functional after immobilization of the N-terminal fragment to a solid support which enables recycling of the immobilized fragment. This strategy enhances overall productivity of N-pro Fusion Technology and thus allows more efficient production of recombinant proteins with reduced costs and in higher yields. Overall, the N-pro complementation system has, depending on the size of the target molecule, potential to increase the productivity up to 4 fold for batch refolding and even more for on-column refolding strategies by the proven possibility of regeneration of the immobilized fragment. (C) 2015 The Authors. Published by Elsevier Inc.
- Authors BOKU Wien:
- Cserjan Monika
- Dürauer Astrid
- Walther Cornelia
- Find related publications in this database (Keywords)
- Recombinant protein production
- Autoprotease N-pro
- Intein
- Protein fragment complementation
- Immobilization
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