University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Amer, H; Hofinger, A; Kosma, P.
(2003):
Synthesis of neoglycoproteins containing O-methylated trisaccharides related to excretory/secretory antigens of Toxocara larvae.
Carbohydr Res. 2003; 338(1):35-45
- Abstract:
- The disaccharides allyl beta-D-galactopyranosyl-(1 --> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 10a and 10b and the trisaccharides allyl 2-O-methyl-alpha-L-fucopyranosyl-(1 --> 2)-beta-D-galactopyranosyl-(1--> 3)-2-acetamido-2-deoxy-beta- and alpha-D-galactopyranoside 18a and 18b have been prepared using stepwise assembly of the sugar units. The glycosidic linkages were formed employing the trichloroacetimidate procedure for the attachment of the galactopyranosyl residue and N-iodosuccinimide/triflic acid activation of an ethyl 1-thiofucopyranoside donor for fucosylation. Deprotection furnished the allyl glycosides which were converted into cysteamine-spacered ligands, activated with thiophosgene and subsequently linked to bovine serum albumin. The neoglycoproteins serve as immunoreagents to determine epitope specificities of monoclonal antibodies directed against highly immunogenic O-glycans located at the surface of Toxocara larvae. (C) 2002 Elsevier Science Ltd. All rights reserved.
- Authors BOKU Wien:
- Hofinger-Horvath Andreas
- Kosma Paul
- BOKU Gendermonitor:
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Antigens, Helminth - chemistry
- Cattle -
- Glycoproteins - chemical synthesis
- Helminth Proteins - chemistry
- Larva - immunology
- Serum Albumin, Bovine - chemistry
- Toxocara - immunology
- Trisaccharides - chemical synthesis
- Find related publications in this database (Keywords)
- neoglycoprotein
- Toxocara
- antigen
- O-glycan
- fucose
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