University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Mayer, H.K..
(2005):
Milk species identification in cheese varieties using electrophoretic, chromatographic and PCR techniques.
International Dairy Journal, 15, 595–604
FullText
FullText_BOKU
- Abstract:
- Different methods were applied to standard mixtures of milk from different species and to model cheeses of different ages to study their applicability for the detection of cows' milk and determination of the percentage of cows' milk in mixed milk cheeses. Urea-polyacrylamide gel electrophoresis (urea-PAGE) and anion-exchange high performance liquid chromatography (HPLC) of caseins was restricted to the control of the adulteration of milk. Isoelectric focusing (IEF) of gamma-caseins according the EU reference method was well suited for detection of cows' milk even in matured cheese, but could not discriminate ewes' milk from goats' milk. IEF and cation-exchange HPLC of para-kappa-caseins were appropriate for differentiation of bovine, ovine and caprine milk. Urea-PAGE and IEF of whey proteins was useful for the species authentication of whey cheeses. Polymerase chain reaction using species-specific primers was a highly sensitive technique for the qualitative detection of cows' milk even in overripe mixed cheese. (c) 2005 Elsevier Ltd. All rights reserved.
- Authors BOKU Wien:
- Mayer Helmut
- Find related publications in this database (Keywords)
- species identifications
- mixed cheese
- urea-PAGE
- isoelectric focusing
- HPLC
- PCR
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