University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Schähs, M; Strasser, R; Stadlmann, J; Kunert, R; Rademacher, T; Steinkellner, H.
(2007):
Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern.
Plant Biotechnol J. 2007; 5(5):657-663
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- Abstract:
- in recent years, plants have become an attractive alternative for the production of recombinant proteins. However, their inability to perform authentic mammalian N-glycosylation may cause limitations for the production of therapeutics. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex Winked glycans, as these N-glycan epitopes are immunogenic in mammals. In our attempts towards the humanization of plant N-glycans, we have generated an Arabidopsis thaliana knockout line that synthesizes complex N-glycans lacking immunogenic xylose and fucose epitopes. Here, we report the expression of a monoclonal antibody in these glycan-engineered plants that carry a homogeneous mammalian-like complex N-glycan pattern without beta 1,2-xylose and core alpha 1,3-fucose. Plant and Chinese hamster ovary (CHO)-derived immunoglobulins (IgGs) exhibited no differences in electrophoretic mobility and enzyme-linked immunosorbent specificity assays. Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform.
- Authors BOKU Wien:
- Kunert Renate
- Stadlmann Johannes
- Steinkellner Herta
- Strasser Richard
- Find related publications in this database (using NML MeSH Indexing)
- Animals -
- Antibodies, Monoclonal - genetics
- Arabidopsis - genetics
- CHO Cells - genetics
- Cricetinae - genetics
- Cricetulus - genetics
- Enzyme-Linked Immunosorbent Assay - genetics
- Epitopes - chemistry
- Gene Deletion - chemistry
- Glycosylation - chemistry
- Humans - chemistry
- Immunoglobulin G - immunology
- Plant Leaves - genetics
- Plants, Genetically Modified - genetics
- Polysaccharides - chemistry
- Recombinant Proteins - immunology
- Find related publications in this database (Keywords)
- antibodies
- beta 1,2-xylose and core alpha 1,3-fucose
- plant glycan
- engineering
- recombinant proteins
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